Solanum khasianum Clarke. (Solanaceae), an important medicinal plant used to treat several diseases. Leaf, root, fruit and bark extracts of S. khasianum and the green synthesized silver nanoparticles were assayed for its antibacterial activity against gram positive and gram negative bacteria, using agar well diffusion method. Among all the solvent extracts tested, methanolic extracts were found to possess elevated activity. The optimal antibacterial activity is exhibited by silver nanoparticles against Pseudomonas flourescens with a maximal zone of inhibition (22mm). In the present study, the efficacy of green synthesized silver nanoparticles over the different plant extracts of S. khasianum against different bacterial strains was reported. This investigation discloses the potentiality of using silver nanoparticles as an antibacterial agent.