Isolation, Screening and Morphological characterization of Laccase producing fungi

Abstract

Research was carried out on the production of extracellular laccase enzyme by fungi isolated from different lignocellulolytic waste soils within the Bhilai-Durg region of Chhattisgarh, India. Soil samples were collected from lignocellulolytic waste such as flower, citrus peel, and paddy straw soil and immediately transported to the laboratory for analysis. Fungal colony was isolated by the serial dilution method on potato dextrose agar. A total of 49 fungal cultures were isolated from the soil samples. Isolated fungal culture were screened for production of laccase enzyme in guaiacol containing potato dextrose agar plate. Laccase producing fungi was morphologically identified by Agharkar Research Institute in Pune. The fungal isolates were identified as Scytalidium lignicola, Humicola fuscoatra, Fusarium sp., Paecilomyces sp., Curvularia sp., Fusarium sp., Aspergillus niger, Chaetomium sp., Penicillium sp., and Histoplasma capsulatum.